100 % herbal ingredient / 0% chemical     

INCENSE STICK  NAMED SWASTHYA DHOOP CHURNA MICROBIAL STUDY IN HOSPITAL ENVIRONMENT

ABSTRACT: -

Hospital buildings may be regarded as dynamic environments affected by season, weather conditions, indoor ventilation system design and operation, intrusion of moisture, outdoor microbial load and the number of occupants, visitors and human activities. Airborne microorganisms, such as bacteria, can originate not only from humans (including patients), but can also be spawned by various indoor hospital characteristics and outdoor environmental sources. These factors may be associated with conditions for microbial growth, leading to bacterial air pollution. Poor hospital indoor air quality may cause hospital-acquired infections, sick hospital syndrome and various occupational hazards. Therefore, ensuring effective disinfection procedures and regular monitoring measures of hospital environment is essential for microbial control by detecting the irregular introduction of airborne particles via clothing of visitors and medical staff or carriage by personal and medical materials.

Stick study: -

MEDIA

(1) Soyabean Casein Digest Agar / Plate count Agar (For bacterial count)

            (2) Sabouraud Dextrose Agar / SDA  (For fungal count )

Reference:-As per USP standards.

procedure

·         Prepare and sterilize agar media as per the instruction given on the label of the respective pack of dehydrated media.

·         After sterilization of the media, pour the media in enough quantity in labeled disposable Petri plates or sterile Petri plates.

·         Allow the plates to solidify. Incubate all the plates at 37°C± 1°C/ 25°C± 1°C overnight to check the sterility of the medium.

·         Discard the plates showing growth of microorganisms.

·         Expose the SCDA/SDA agar plates at various sites for about 4 hr’s before study in different environment.

·         After completion of 4 hr’s start dhoop in room for 30 mins. of 30 mins. (Stick dose 6 gm during fogg).

·         Fogg is continuous removed again exposed plate in same locatation.

·         Again, immediately expose the SCDA/SDA agar plates for 4 hr’s.

·         Incubate SCDA/SDA in inverted position for three days at 37°C± 1°C. SCDA/SDA plates are incubated for 5-7days at 25^oC± 1°C.

·         Keep one plate of both the medium as control. Control plates should not show any colony after completion of incubation period.

·         After completion of incubation period count the colonies appearing on the plates and express the result as Number of CFU /plate.

                                                              OBSERVATION DATA SHEET

AIR MONITORING

Date of analysis                                                                : 18 may 2019

Media Used and Batch No                           : SCDA and SDA

Time of plate exposure                                 :  4 hr’s

Temperature of Incubation                        :  3-5 days

Date & Time of Incubation                          :  18 MAY 2019 10:AM

Date & Time of Result (TPC)        : 21 MAY 2019 9:00 AM

Date & Time of Result (YMC)      : 23 MAY 2019 9:00 AM

OBSERVATION DATA SHEET

AIR MONITORING

Date of analysis                                             : 15 june 2019

Media Used and Batch No                           : SCDA and SDA

Time of plate exposure                                : 4 hr’s.

Temperature of Incubation                         : 3-5 days

Date & Time of Incubation                           : 15 June 2019 10:AM

Date & Time of Result (TPC)        : 18 June 2019 (9:00 AM)

Date & Time of Result (YMC)      : 20 June 2019 (9:00 AM)

CONCLUSION - during this study use 6 gm dose used for bacteria and fungus identification. instence dhoop stick kill the certain types of bacteria and fungus and clean the atmospheric air.